Monolith Immuno-spotting Multiplex Immunosensors in a Microfluidic Device

Here we demonstrate for the first time, a novel fabrication method to integrate microarray spotting technology with in-situ fabricated highly porous polymer monoliths in a microfluidic platform as a high-thourouput multiplex biosensor. Parallel screening of low-abundance analytes from a complex fluidic sample can be realized by covalent linkage of various capture antibodies in discretely defined detection regions within a single integrated monolith by microarray spotting. The low-cost multiplex biosensor is intended for use in resource-poor settings and point-of-care diagnostic applications.